BIOTECHNOLOGICAL METHOD OF PROPAGATION OF PAULOWNIA SSP

Abstract

The purpose of this study is to develop highly efficient methods of in vitro propagation of introduced species of the genus Paulownia Siebold & Zucc. for the development of bioenergy in Ukraine, obtaining a collection of raw materials for selection, replication of high-quality planting material. According to the results of the experimental studies, it was established that the germination of seeds in vivo took place for 14 days, the sterilization of the germ leaves and apical meristems was carried out with solutions of sodium hypochlorite with a mass fraction of 20% and an exposure time of 2–5 minutes. Additional shoots were obtained on Murashige-Skuga nutrient medium with the inclusion of hormonal substances BAP 1.5 mg/dm3 in order to achieve the maximum number of internodes and rooting of cultural seedlings, nutrient media containing BAP 0.3 mg/dm3 and kinetin 0.15 mg/dm3 were added. A method of microclonal reproduction of paulownia was developed using as explants apical meristems from seed seedlings of introduced species of the genus Paulownia Siebold & Zucc. and the obtained collection of starting materials for in vitro breeding (Paulownia tomentosa Steud., Paulownia elongata S.Y.Hu; Paulownia '9501' (Paulownia tomentosa × Paulownia fortunei); Paulownia 'Zo7' (Paulownia tomentosa × Paulownia fortunei × Paulownia kawakamii); Paulownia 'Shan thong' (Paulownia tomentosa × Paulownia fortunei). The highest percentage of shoot regeneration on the medium with the addition of 1.5 mg/dm3 kinetin and 1.5 mg/dm3 BAP was characteristic of selected lines of Paulownia elongata S.Y.Hu with a shoot formation coefficient of 2 pp./ex and 'Z07' (P. fortunei x P. tomentosa x P. kawakami) with a value of 2.7 pg./ex. After transplanting to nutrient medium with BAP 0.3 mg/dm3 and gibberellic acid 0.15 mg/dm3 the best results were in Paulownia tomentosa Steud., with the formation of 7.4 shoots per plant.